1. A. Personal statement.

My research is focused in studying enzymatic and non-enzymatic lipid oxidation, in particular those derived from arachidonic acid (AA). Analysis involve different methodologies while in the last years our focus is the post-traslational modifications of proteins by AA-derived nitrated products (nitroarachidonic acid, NO2-AA) as well as the lipidomic studies from biological samples by LC-MS/MS. I have chemically characterized different isomers of NO2-AA, how these products affect the activation of different cell types, e.g. platelets and macrophages as well as the mechanism of inhibition of the AA-metabolizing enzyme cyclooxygenase (COX). More recently we were focused in the modulation of the production of reactive oxygen species by NADPH oxidase (NOX) by the nitrated products, the mechanism of reaction involved  and  in  particular  the  effects  of  NO2-AA  on  Protein  Disulfide  Isomerase  (PDI),  a  protein necessary for NOX activation at the cell membrane. NO2-AA is able to reversible covalent modify Cys residues at the active site of the protein, inhibiting it and affecting reactive species formation. All these studies involved mass spectrometry and LC-MS/MS studies. Overall, during the last 10 years I reached experience in measuring reactive species formation in different cell types, analyzing the mechanisms of action of lipid derived products leading to this effect. In adddtion, and of importance for this proposal, we gained  experience  in  using  Mass  Spectrometry  for  lipid  analysis  and  their  derived  effects. At  this moment, lipidomic studies are being applied in different cell and animal models to correlate the levels of lipid-derived products with the onset, progression and modulation of diseases or drug treatments.

  1. B. Positions and Honors.

Positions and Employment

2016 – Adjunct Vistant Professor of Physiological Department, Federal University of Maranhao, Sao Luis, Maranhao-Brazil

2013 – Adjunct Associate Professor of Pharmacology and Chemical Biology, University of Pittsburgh

2010-date  Associate  Professor  of  Biochemistry,  Department  of  Biochemistry-School  of  Medicine, Universidad de la República, Montevideo, Uruguay

2002  -2010  Assistant  Professor  of  Biochemistry,  Department  of  Biochemistry-School  of  Medicine, Universidad de la República, Montevideo, Uruguay

1998-2002 Instructor of Biochemistry, Department of Biochemistry-School of Medicine, Universidad de la

República, Montevideo, Uruguay

Other Experience and Professional Memberships

2014-         Council member of the program of postgraduate studies in Uruguay (PEDECIBA)

2011-2013 President of the Society of Biochemistry and Molecular Biology (SBBM)-Uruguay

2009-2011 Council Member, Society of Biochemistry and Molecular Biology (SBBM)-Uruguay

2007          Member of the Local Organizing Committee of the Free Radicals meeting in Montevideo


2015          Roberto Caldeyro Barcia Award in Biology, PEDECIBA, Uruguay

2013          Fellowship of the National Investigators System- Education Ministry, Uruguay

2012          Fellowship of the IUBMB-Young Scientist Program

2011          Fellowship of the National Investigators System- Education Ministry, Uruguay

2011          Research Mini-Fellowship of the SFRBM, USA

2010          Travel Award- 17th Annual Meeting of the SFRBM, USA

2009          Fellowship of the National Investigators System- Education Ministry, Uruguay

2008-date  Researcher of Basic Sciences Development Program-PEDECIBA (Biology and Chemistry), Uruguay

2009          Roberto Caldeyro Barcia Award, PEDECIBA-PNUD, Uruguay

2008          EAS Travel Grant, European Atherosclerosis Society

2007          Young  Investigator  Award,  V  Meeting  of  the  SFRBM-  South  American  Group  and  V International Conference on Peroxynitrite and Reactive Nitrogen Species, Uruguay

2007          XI Prize Young Talent Award in Life Sciences, 37th Meeting  of the SBBq-10th IUBMB Conference, Brazil

2006          Award of the Uruguayan Society of Biosciences for Young Scientists

2005          Young Investigator Award, IV Meeting of the South American Group of the SFRBM- Brazil

2004          Young Investigator Award – University of  Alabama, 12th Biennial  Meeting of the SFRI- Argentina

2004          Fellowship of  the  Fondo Nacional de Investigadores-Ministerio  de  Educación  y Cultura, Uruguay

2004          Ph.D Research fellowship of PEDECIBA-School of Chemistry, Uruguay

2002          Travel Award- 9th Annual Meeting of The Oxygen Society, USA

2001          M.Sc. Research fellowship of PEDECIBA-School of Chemistry, Uruguay

Honors and Professional Memberships

PEDECIBA Researcher (Program for the Development of Basic Sciences; PEDECIBA-Uruguay). (since 2008)

Investigator, Level 1. National Research System, Uruguay (since 2008)

Investigator, Level 1. National Research Fund, Ministerio de Educación y Cultura, Uruguay (2004)

  1. C. Peer-reviewed publications (in chronological order, in the last 5 years).
  2. ANDRÉS TROSTCHANSKY; HOMERO RUBBO. Anti-inflammatory signaling actions of electrophilic nitro-arachidonic acid in vascular cells and astrocytes. Archives of Biochemistry and Biophysics, v.: 617, p.: 155 – 161, 2017.
  1. INéS MARMISOLLE; JENNYFER MARTíNEZ; JIE LIU; MAURICIO MASTROGIOVANNI; MARíA M. FERGUSSON; ILSA I. ROVIRA; LAURA CASTRO; ANDRÉS TROSTCHANSKY; MARíA MORENO; LIU CAO; TOREN FINKEL; CELIA QUIJANO. Reciprocal regulation of acetyl-CoA carboxylase 1 and senescence in human fibroblasts involves oxidant mediated p38 MAPK activation. Archives of Biochemistry and Biophysics, v.: 613, p.: 12 – 22, 2017.
  1. H. R.  SOUSA;  R.  S.  GASPAR;  E.  M.  L.  SENA;  SAMIRA  ABDALLA  DA  SILVA;  J.  L.  DE  L. FONTELLES; T. L. S. ARAúJO; MAURICIO MASTROGIOVANNI; D. M. FRIES; A.P.S. AZEVEDO- SANTOS; FRANCISCO LAURINDO; ANDRÉS TROSTCHANSKY; ANTONIO MARCUS DE ANDRADE PAES. Novel antiplatelet role for a protein disulfide isomerase-targeted peptide: Evidence of covalent binding to C-terminal CGHC redox motif. Journal of Thrombosis and Haemostasis, v.: 15 4, p.: 774 – 784, 2017.
  1. LUCíA GONZáLEZ-PERILLI; MAURICIO MASTROGIOVANNI; DENISE DE CASTRO FERNANDES; HOMERO RUBBO; FRANCISCO LAURINDO; ANDRÉS TROSTCHANSKY. Nitroarachidonic acid (NO2-AA) inhibits Protein Disulfide Isomerase (PDI) through reversible covalent adduct formation with critical cysteines. Biochimica et Biophysica Acta-General Subjects, v.: 1861, p.: 1131 – 1139, 2017.
  1. CELIA QUIJANO; LAURA CASTRO; MADIA TRUJILLO; ANDRÉS TROSTCHANSKY. Interplay between oxidant species and energy metabolism. Redox Biology, v.: 8, p.: 28 – 42, 2016
  2. BEATRIZ SANCHEZ-CALVO; ADRIANA M. CASSINA; NATALIA RíOS; A. GONZALO PELUFFO; JOSÉ BOGGIA; RAFAEL RADI; HOMERO RUBBO; ANDRÉS TROSTCHANSKY. Nitro-Arachidonic Acid Prevents Angiotensin II-Induced Mitochondrial Dysfunction in a Cell Line of Kidney Proximal Tubular Cells. PLoS ONE, v.: 11 3, 2016.
  1. PABLO DIAZ-AMARILLA*; ERNESTO MIQUEL*; ANDRÉS TROSTCHANSKY*; EMILIANO TRIAS; ANA M. FERREIRA; BRUCE A. FREEMAN; PATRICIA CASSINA; LUIS BARBEITO; MARCELO VARGAS; HOMERO RUBBO. Electrophilic nitro-fatty acids prevent astrocyte-mediated toxicity to motor neurons in a cell model of familial amyotrophic lateral sclerosis via nuclear factor erythroid 2- related factor activation.. Free Radical Biology and Medicine, v.: 95, p.: 112 – 120, 2016 *Pablo Diaz-Amarilla, Ernesto Miquel y Andrés Trostchansky equally contributed to the work.
  1. RENATO GASPAR SOARES; ANDRÉS TROSTCHANSKY; ANTONIO MARCUS DE ANDRADE PAES. Potential  role  of  protein  disulfide  isomerase  in  metabolic  syndrome-derived  platelet hyperactivity. Oxidative Medicine and Cellular Longevity, v.: 2016, 2016.
  1. MARCO  FAZZARI*;   ANDRÉS   TROSTCHANSKY*;   FRANCISCO   J.   SCHOPFER;   SONIA SALVATORE; BEATRIZ SANCHEZ-CALVO; DARIO VITTURI; RAQUEL VALDERRAMA; JUAN B. BARROSO; RAFAEL RADI; HOMERO RUBBO. Olives and Olive Oil are Sources of Electrophilic Fatty Acid Nitroalkenes. PLoS ONE, v.: 9 1, 2014 * Andres Trostchansky y Marco Fazzari equally contributed to the work.
  1. ANDRÉS TROSTCHANSKY; LUCÍA BONILLA; LUCÍA GONZALEZ; HOMERO RUBBO. Nitro-fatty acids: Formation, Redox Signaling and Therapeutic Potential . Antioxidants & redox signaling, v.: 19 11, p.: 1257 – 1265, 2013.
  1. LUCÍA GONZALEZ; MARÍA N. ALVAREZ; CAROLINA PROLO; RAFAEL RADI; HOMERO RUBBO; ANDS  TROSTCHANSKY.  Nitroarachidonic  acid  prevents  NADPH  oxidase  assembly  and superoxide radical production in activated macrophages.. Free Radical Biology and Medicine, v.: 58, p.: 126 – 133, 2013.
  1. LUCÍA BONILLA; VALERIE  B.  O´DONNELL;  STEPHEN  CLARK;  HOMERO  RUBBO;  ANDRÉS TROSTCHANSKY.  Regulation  of  protein  kinase  C  by  nitroarachidonic  acid:  Impact  on  human platelet activation. Archives of Biochemistry and Biophysics, v.: 533, p.: 55 – 61, 2013.

Book chapters in the last 5 years (in chronological order)

  1. HOMERO RUBBO;  LUCÍA  GONZALEZ;  MAURICIO  MASTROGIOVANNI;  BEATRIZ  SANCHEZ CALVO; ANDRÉS TROSTCHANSKY. Nitro-arachidonic acid:downstream signaling and therapeutics , 2016.BIOCHEMISTRY OF OXIDATIVE STRESS. Physiopathology and Clinical Aspects. v.: 1 , 1 , 16, p.: 79 – 93. Editors: Ricardo J. Gelpi, Alberto Boveris and Juan J. Poderoso, Editorial: Springer , Ney York.
  2. BRUCE A. FREEMAN; MICHAELA PEKAROVA; HOMERO RUBBO; ANDRÉS TROSTCHANSKY. Electrophilic Nitro-Fatty Acids: Nitric Oxide and Nitrite-Derived Metabolic and Inflammatory Signaling Mediator , 2016. Nitric Oxide Biology and Pathology. v.: 3, Editor: Loui Ignarro. Editorial: Elsevier.
  1. HOMERO RUBBO; ANDRÉS TROSTCHANSKY. Nitro-fatty acids: Detection, characterization and signaling in olives and olive oils , 2013. Role of Nitric Oxide in Plants under Adverse Environmental Conditions.Editors: Dr. M. Nasir Khan; Prof. Firoz Mohammad; Dr. Francisco J. Corpas. Editorial: SPRINGER/ELSEVIER.
  1. ANDRÉS TROSTCHANSKY; HOMERO RUBBO. Formation of nitrated lipids and their biological relevance , 2013. Lipid Oxidation in Health and Disease. Editorial: Taylor and Francis Publishers , Boca Raton.


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